Goncharskaia T Ia, Koroleva V G, Umnova L V, Bogomolova N S, Treskina O S
Antibiotiki. 1981 May;26(5):367-70.
A modification of the microbiological agar-diffusion method for rapid determination of gentamicin, sisomycin and kanamycin levels in the blood serum of patients is described. The decrease in the time for determination of the antibiotic levels in the serum specimens with the modified method was provided by the use of a higher inoculation dose of the test microbe, higher levels of the incubation temperature and an enriched nutrient medium. The assay time was decreased from 18 to 3--4 hours as compared to the routine agar-diffusion method.
本文描述了一种用于快速测定患者血清中庆大霉素、西索米星和卡那霉素水平的微生物琼脂扩散法的改良方法。通过使用更高接种剂量的测试微生物、更高的培养温度和富集营养培养基，改良后的方法缩短了血清标本中抗生素水平的测定时间。与常规琼脂扩散法相比，测定时间从18小时减少到3 - 4小时。
