Laskov R, Kim J K, Woods V L, McKeever P E, Asofsky R
Eur J Immunol. 1981 Jun;11(6):462-8. doi: 10.1002/eji.1830110605.
Four cell lines derived from spontaneous BALB/c lymphoma tumors were analyzed with regard to the type of their membrane immunoglobulins (Ig). Using lactoperoxidase iodination of membrane proteins combined with immunoprecipitation and electrophoresis on polyacrylamide gel, three of these cell lines (X16c, L10A and K46) were found to express the monomeric form of IgM and IgD as well as half molecules. One cell line (M12) lacked both IgM and IgD. The apparent mol. wt of the lymphoma micro chain was about 80 000 and exceeded the mol. wt. of 75 000 determined for micro chains secreted by myeloma cells. The mol. wt. of the delta heavy chain was found to be 66 000. Immunofluorescence showed that the L10A and X16c lines expressed lambda light chains on their cell surface. Another Ig-bearing cell line (K46) expressed both lambda and kappa chains. Thus, three out of the four B lymphomas examined expressed both IgM and IgD with light chains of the Lambda type. These results, together with our previous findings which demonstrate the presence of Ia and Fc receptors on the same cells, indicate that spontaneous B lymphomas in BALB/c mice are the malignant counterpart of mature B lymphocytes.
对源自自发形成的BALB/c淋巴瘤肿瘤的四种细胞系进行了膜免疫球蛋白（Ig）类型分析。通过膜蛋白的乳过氧化物酶碘化结合免疫沉淀以及聚丙烯酰胺凝胶电泳，发现其中三种细胞系（X16c、L10A和K46）表达IgM和IgD的单体形式以及半分子。一种细胞系（M12）既缺乏IgM也缺乏IgD。淋巴瘤微链的表观分子量约为80000，超过了骨髓瘤细胞分泌的微链所确定的75000的分子量。发现δ重链的分子量为66000。免疫荧光显示L10A和X16c细胞系在其细胞表面表达λ轻链。另一个带有Ig的细胞系（K46）同时表达λ链和κ链。因此，所检测的四种B淋巴瘤中有三种表达IgM和IgD以及λ型轻链。这些结果，连同我们之前证明同一细胞上存在Ia和Fc受体的发现，表明BALB/c小鼠中的自发B淋巴瘤是成熟B淋巴细胞的恶性对应物。
