酿酒酵母中同宗配合基因HML和HMR的精确图谱绘制。
Precise mapping of the homothallism genes HML and HMR in Saccharomyces cerevisiae.
作者信息
Klar A J, McIndoo J, Hicks J B, Strathern J N
出版信息
Genetics. 1980 Oct;96(2):315-20. doi: 10.1093/genetics/96.2.315.
Abstract
The HML and HMR loci carry unexpressed copies of MATa and MAT alpha information, and a replica of that information is transposed to MAT during mating-type interchange in Saccharomyces yeasts. A negative control mechanism keeps silent the information located at the HML and HMR loci. We mapped these loci by constructing strains in which these loci are expressed. In these strains, the mating type of the segregants is dependent upon the allele at HML and HMR. This novel approach is independent of their switching function. HML is located on the left arm of chromosome III distal to his4 by about 26.8 centimorgans (cM). HMR maps on the right arm of the same chromosome distal to thr4 by about 39.8 cM and proximal to MAL2 by about 1.0 cM. The results allow the exact placement of these loci and are in accord with the observations made by Harashima and Oshima (1976).
摘要
HML和HMR位点携带MATa和MATα信息的未表达拷贝，在酿酒酵母交配型转换过程中，该信息的一个副本会转移到MAT位点。一种负调控机制使位于HML和HMR位点的信息保持沉默。我们通过构建使这些位点表达的菌株来对这些位点进行定位。在这些菌株中，分离株的交配型取决于HML和HMR位点的等位基因。这种新方法独立于它们的转换功能。HML位于第三条染色体左臂上，距离his4约26.8厘摩（cM）。HMR位于同一条染色体右臂上，距离thr4约39.8 cM，距离MAL2约1.0 cM。这些结果确定了这些位点的确切位置，与原岛和大岛（1976年）的观察结果一致。
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