酿酒酵母III号染色体左端粒有一个X型而非Y'型自主复制序列（ARS）区域。
The Saccharomyces cerevisiae chromosome III left telomere has a type X, but not a type Y', ARS region.
作者信息
Button L L, Astell C R
出版信息
Mol Cell Biol. 1986 Apr;6(4):1352-6. doi: 10.1128/mcb.6.4.1352-1356.1986.
A yeast Saccharomyces cerevisiae telomeric region was isolated by chromosome walking from HML alpha, the most distal known gene on the chromosome III left (IIIL) end. The terminal heterodisperse 3.3-kilobase (kb) SalI fragment on chromosome IIIL, 8.6 kb distal to HML alpha, was cloned in a circular vector to generate a telomeric probe. Southern hybridization and DNA sequencing analyses indicated that 0.6 kb (+/- 200 base pairs) of 5'-C1-3A-3' simple tandem repeat sequence, adjacent to a 1.2-kb type X ARS region, constitutes the telomere on the chromosome IIIL end, and no type Y' ARS region homologies exist between HML alpha and the IIIL terminus.
通过染色体步移从HMLα（位于III号染色体左臂（IIIL）末端已知的最远端基因）分离出酿酒酵母的端粒区域。在III号染色体左臂上，HMLα远端8.6 kb处的3.3千碱基（kb）末端异质SalI片段被克隆到一个环状载体中，以产生一个端粒探针。Southern杂交和DNA测序分析表明，与一个1.2 kb的X型自主复制序列（ARS）区域相邻的0.6 kb（±200个碱基对）的5'-C1-3A-3'简单串联重复序列构成了IIIL染色体末端的端粒，并且HMLα与IIIL末端之间不存在Y'型ARS区域同源性。
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