Herrmann W, Schütz C, Reuter W
Z Gesamte Inn Med. 1983 Jan 1;38(1):17-22.
For the clinical practice methods of the determination of HDL-cholesterol made their way which are based on the precipitation of apolipoprotein-B-containing lipoproteins and a determination of cholesterol following. The expensive methods of the ultracentrifugation serve as reference methods. The most-spread precipitation techniques (heparin/MCl2, dextran sulphate/CaCl2 or MgCl2 photungstic acid/MgCl2) are comparatively observed with regard to their effectiveness, practicability and methodical and technical conditions (influence of the concentration of the precipitation reagents, pH-value, temperature, incubation and centrifugation conditions). Results of own investigations as well as data from literature are presented to the problem of the harmonization of the cholesterol determination with the precipitation technique. According to the opinion of the authors for the enzymatic determination of cholesterol by means of the CHOD-PAP-method the phosphotungstic acid precipitation well stood the test, whereas for the chemical determination of cholesterol after Liebermann-Burchard in manual or automatized works the precipitation by means of dextran sulphate/CaCl2 (40 g/l, 2.0 mol/l) is to be recommended. The superabundant precipitations with phosphotungstic acid and dextran sulphate/MgCl2 (20 g/l, 2.0 mol/l) achieve higher results in Liebermann-Burchard's reaction likely on account of interferences.
对于基于含载脂蛋白B的脂蛋白沉淀及后续胆固醇测定的高密度脂蛋白胆固醇临床检测方法，其已经逐步发展起来。昂贵的超速离心法用作参考方法。对最常用的沉淀技术（肝素/氯化锰、硫酸葡聚糖/氯化钙或氯化镁、磷钨酸/氯化镁）在有效性、实用性以及方法和技术条件（沉淀试剂浓度、pH值、温度、孵育和离心条件的影响）方面进行了比较观察。展示了自身研究结果以及文献数据，以探讨胆固醇测定与沉淀技术的协调问题。根据作者观点，对于采用CHOD - PAP法进行胆固醇的酶法测定，磷钨酸沉淀经得住考验，而对于在手工或自动化操作中按照Liebermann - Burchard法进行胆固醇的化学测定，推荐采用硫酸葡聚糖/氯化钙（40 g/l，2.0 mol/l）沉淀法。磷钨酸和硫酸葡聚糖/氯化镁（20 g/l，2.0 mol/l）的过量沉淀在Liebermann - Burchard反应中可能由于干扰而得到更高结果。
