Smith R J, Fisher J W
J Pharmacol Exp Ther. 1976 Jun;197(3):714-22.
The generation of erythropoietin purportedly involves the interaction of an enzyme, of renal origin, and a circulating plasma protein substrate. Cobalt, which has the capacity to stimulate erythropoietin formation, was evaluated for its effects on renal and plasma protease activity; in addition, the nature of the erythrogenic mechanism was investigated. Rats receiving one s.c. injection of cobalt demonstrated significant increases in activity of renal cathepsins A and B. The activity of a plasma protease was also elevated in rats after cobalt treatment. Cathepsins A and B and plasma protease were sensitive to the same enzymatic inhibitory agents, and all three enzymes demonstrated activity patterns similar to that of the renal erythropoietic factor [enzyme(s) presumed responsible for generating erythropoietin] in rats treated with cobalt. These data may help to elucidate the mechanism of erythropoiesis.
据称，促红细胞生成素的产生涉及一种源自肾脏的酶与一种循环血浆蛋白底物之间的相互作用。对具有刺激促红细胞生成素形成能力的钴，评估了其对肾脏和血浆蛋白酶活性的影响；此外，还研究了促红细胞生成机制的性质。接受一次皮下注射钴的大鼠，其肾脏组织蛋白酶A和B的活性显著增加。钴处理后的大鼠血浆蛋白酶活性也有所升高。组织蛋白酶A和B以及血浆蛋白酶对相同的酶抑制剂敏感，并且在用钴处理的大鼠中，这三种酶的活性模式与肾脏促红细胞生成因子（推测负责产生促红细胞生成素的酶）的活性模式相似。这些数据可能有助于阐明红细胞生成的机制。
