Properties of reconstituted rabbit pulmonary mixed-function oxidase system with cytochrome P-450.

Reconstituted rabbit pulmonary mixed-function oxidase (MFO) system is composed of four components: NADPH cytochrome P-450 reductase, cytochrome P-450, phosphatidylcholine and cholate. Evidence has been presented that phosphatidylcholine and cholate form micelles that take an active part in the reconstitution. Order of addition of MFO system components in the reconstitution has been intensively studied. Several titration curves well describe reconstituted system. Experimental data suggest formation of dissociable complex of reductase, cytochrome P-450 and micelles A model for reconstituted rabbit pulmonary MFO system is discussed.