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迈向细胞色素P-450重组混合功能氧化酶系统的标准化。

Towards standardization of the reconstituted mixed function oxidase systems with cytochrome P-450.

作者信息

Szutowski M M

出版信息

Arch Toxicol Suppl. 1980;4:385-7. doi: 10.1007/978-3-642-67729-8_84.

DOI:10.1007/978-3-642-67729-8_84
PMID:6933943
Abstract

Reconstituted rabbit pulmonary mixed function oxidase (MFO) system with cytochrome P-450 is composed of NADPH cytochrome P-450 reductase, cytochrome P-450, phospholipid extract and cholate. Factors essential for the reconstitution of the active system include the component concentrations and ratios, preincubation and the ionic strength of the buffer. The rabbit pulmonary MFO system can be separated, purified and reconstituted with good reproducibility. However there are many factors influencing reconstituted activity mainly associated with the reconstitution and reductase purification procedure.

摘要

由细胞色素P - 450组成的重组兔肺混合功能氧化酶（MFO）系统由NADPH细胞色素P - 450还原酶、细胞色素P - 450、磷脂提取物和胆酸盐组成。活性系统重组所必需的因素包括组分浓度和比例、预孵育以及缓冲液的离子强度。兔肺MFO系统可以进行分离、纯化和重组，且具有良好的重现性。然而，有许多影响重组活性的因素，主要与重组和还原酶纯化过程有关。

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