Effect of sodium cholate on the NADPH cytochrome P-450 reductase activity in the reconstituted rabbit pulmonary mixed-function oxidase system.

Solubilization, separation and purification procedures for rabbit pulmonary mixed-function oxidase (MFO) system have been shown to influence the NADPH cytochrome P-450 reductase component activity in the reconstituted system. The reductase activity as judged by cytochrome c reduction does not correlate with the reductase ability to interact with other MFO system components. Sodium cholate used in several solubilization and purification procedures may alter the reconstituted drug metabolizing activity.