Nouws J F
Vet Q. 1981 Jul;3(3):136-42. doi: 10.1080/01652176.1981.9693814.
The sensitivity of microbiological assay methods for sulfonamides in muscle, kidney, serum, and milk was improved with Standard II Nähragar (Merck 7883), the medium being supplemented with trimethoprim, 1% sodium chloride, and 0.4% dextrose. The agar was seeded with 10(4) spores of Bacillus subtilis BGA per ml medium. The trimethoprim concentrations per ml agar were, at pH 6.20, pH 7.0, and pH 8.0 respectively 0.30, 0.15 and 0.07 microgram. The sulfonamide sensitivity of the test method depended on the pK-value of the drug tested and on the pH of the agar. For sulfonamides tested in muscle homogenate the optimum sensitivity of the bioassay methods was in the range of 0.32-0.63 microgram/ml; in kidney homogenate it was 0.32-0.63 microgram/ml; in serum 0.32-0.63 microgram/ml; and in milk 0.08-0.32 microgram/ml. The sensitivity for dapsone ranged in these substrates from 0.01 to 0.02 microgram/ml. The assay methods here developed may be used for both qualitative detection and quantitative determinations of sulfonamides in animal tissues, serum, and milk.
采用标准II营养琼脂（默克7883），并在培养基中添加甲氧苄啶、1%氯化钠和0.4%葡萄糖，可提高肌肉、肾脏、血清和牛奶中磺胺类药物微生物检测方法的灵敏度。每毫升培养基接种10⁴个枯草芽孢杆菌BGA孢子。在pH 6.20、pH 7.0和pH 8.0时，每毫升琼脂中甲氧苄啶的浓度分别为0.30、0.15和0.07微克。该检测方法对磺胺类药物的灵敏度取决于被测药物的pK值和琼脂的pH值。对于在肌肉匀浆中检测的磺胺类药物，生物检测方法的最佳灵敏度范围为0.32 - 0.63微克/毫升；在肾脏匀浆中为0.32 - 0.63微克/毫升；在血清中为0.32 - 0.63微克/毫升；在牛奶中为0.08 - 0.32微克/毫升。在这些基质中，氨苯砜的灵敏度范围为0.01至0.02微克/毫升。这里开发的检测方法可用于动物组织、血清和牛奶中磺胺类药物的定性检测和定量测定。
