De Santis E P, Mazzette R
Istituto di Ispezione degli Alimenti di O. A., Università di Sassari.
Boll Soc Ital Biol Sper. 1991 Jun;67(6):561-8.
Sensitivity of Bacillus subtilis BGA and Bacillus stearothermophilus var. calidolactis disc assays to 53 chemio-antibiotics was tested. Test-microorganisms were sown in two different mediums: PM Indicator Agar, Difco U.S.A., and Standard II Nutrient Agar, Merck Germany, modified according to Nouws. The mediums were used with or without addition of Trimethoprim (at a concentration of 0.12 or 0.024 mcg/ml of medium for B. subtilis and for B. stearothermophilus respectively). B. stearothermophilus did not grow in Standard II. However, the B. subtilis assay gave the best results with Standard II, apart for aminoglycosides. The B. stearothermophilus disc assay was the most sensitive to penicillins (Minimum Inhibiting Concentration in mcg/ml, MIC, between 0.001 and 0.004), cephalosporins (MIC between 0.003 and 0.09, apart from Ceftazidime, 0.3) and aminoglycosides (MIC between 0.03 and 0.6). The B.subtilis disc assay showed better sensitivity to quinolines (MIC between 0.05 and 4) and to some tetracyclines (oxytetracycline and chlortetracycline, MIC 0.03). Trimethoprim, where added, determined a higher sensitivity to sulfonamides (MIC between 0.025 and 0.25).
测试了枯草芽孢杆菌BGA和嗜热脂肪芽孢杆菌变种卡利多尔乳酸盘法对53种化学抗生素的敏感性。测试微生物接种在两种不同的培养基中：美国Difco公司的PM指示琼脂和德国默克公司根据努斯改良的标准II营养琼脂。培养基添加或不添加甲氧苄啶（分别以0.12或0.024微克/毫升培养基的浓度用于枯草芽孢杆菌和嗜热脂肪芽孢杆菌）。嗜热脂肪芽孢杆菌在标准II中不生长。然而，除氨基糖苷类外，枯草芽孢杆菌检测在标准II中给出了最佳结果。嗜热脂肪芽孢杆菌盘法对青霉素（最低抑菌浓度以微克/毫升计，MIC，在0.001至0.004之间）、头孢菌素（MIC在0.003至0.09之间，除头孢他啶为0.3）和氨基糖苷类（MIC在0.03至0.6之间）最为敏感。枯草芽孢杆菌盘法对喹啉（MIC在0.05至4之间）和一些四环素（土霉素和金霉素，MIC 0.03）显示出更好的敏感性。添加甲氧苄啶后，对磺胺类药物的敏感性更高（MIC在0.025至0.25之间）。
