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Comparison of different agar diffusion methods for the detection of residues in the kidneys of pigs treated with antimicrobial drugs.用于检测抗菌药物治疗猪肾脏中残留物的不同琼脂扩散法的比较
Meat Sci. 1983;9(4):291-304. doi: 10.1016/0309-1740(83)90039-6.
2
Comparison of different agar diffusion methods for the detection of antimicrobial residues in slaughter animals.用于检测屠宰动物中抗菌药物残留的不同琼脂扩散法的比较。
Acta Vet Scand. 1982;23(3):407-15. doi: 10.1186/BF03546792.
3
Microbiological assay methods for sulfonamides in animal tissues, serum, and milk.动物组织、血清和牛奶中磺胺类药物的微生物测定方法。
Vet Q. 1981 Jul;3(3):136-42. doi: 10.1080/01652176.1981.9693814.
4
An improved bacteriological method for the detection of sulfonamide residues in food.一种用于检测食品中磺胺类药物残留的改良细菌学方法。
Acta Vet Scand. 1976;17(4):458-64. doi: 10.1186/BF03547901.
5
Lysozyme and its presence in bovine milk and serum.溶菌酶及其在牛乳和血清中的存在情况。
Tijdschr Diergeneeskd. 1978 Apr 15;103(8):445-50.
6
Heat stable inhibitors produced in raw milk.生乳中产生的热稳定抑制剂。
Nord Vet Med. 1977 Oct;29(10):436-9.

牛奶中的抗菌残留。不同琼脂扩散法的比较。

Antimicrobial residues in milk. Comparison of different agar diffusion methods.

作者信息

Haapoja A, Korkeala H

出版信息

Acta Vet Scand. 1984;25(2):250-9. doi: 10.1186/BF03547269.

DOI:10.1186/BF03547269
PMID:6439018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8287430/
Abstract

Different agar diffusion methods were compared in order to find a sensitive method for the detection of various antimicrobial residues in milk. A total of 588 producer milk samples were analyzed using subsets of the most sensitive methods. With the IDF method, 2 positive cases (0.34 %) appeared among the producer milk samples, with the Thermocult method 13 positive cases (2.21 and with the Test agar pH 8 method with trimethoprim and glucose 4 positive cases (0.68 %). A combination of the IDF method and the Test agar pH 8 method resulted in 6 positive cases (1.02 and a combination of the Thermocult method and the Test agar pH 8 method in 17 positive cases (2.89 With penicillinase 41 of the positive cases were identified as β-lactam antibiotics and with p-aminobenzoic acid 18 % of the positive cases were identified as sulphonamides. 41 of the positive cases remained unexplained. The best combination for the detection of antimicrobial agents in milk seems to be that of the Thermocult method and the Test agar pH 8 method with trimethoprim and glucose.

摘要

为了找到一种检测牛奶中各种抗菌残留的灵敏方法，对不同的琼脂扩散法进行了比较。使用最灵敏方法的子集对总共588份生产商用牛奶样本进行了分析。采用国际乳品联合会（IDF）方法时，生产商用牛奶样本中出现了2例阳性病例（0.34%）；采用嗜热菌培养基法时，有13例阳性病例（2.21%）；采用含甲氧苄啶和葡萄糖的pH 8测试琼脂法时，有4例阳性病例（0.68%）。IDF方法和pH 8测试琼脂法相结合产生了6例阳性病例（1.02%），嗜热菌培养基法和pH 8测试琼脂法相结合产生了17例阳性病例（2.89%）。使用青霉素酶时，41%的阳性病例被鉴定为β-内酰胺类抗生素，使用对氨基苯甲酸时，18%的阳性病例被鉴定为磺胺类药物。41%的阳性病例原因不明。检测牛奶中抗菌剂的最佳组合似乎是嗜热菌培养基法和含甲氧苄啶和葡萄糖的pH 8测试琼脂法。