Jonas L
Acta Histochem. 1981;68(2):238-47.
Carbonic anhydrase activity was demonstrated in the flask cells of intermediate segments and collecting tubules of the clawfrog-mesonephros with the cobalt-bicarbonate method of Häusler (1958) and Hansson (1967). Additional the luminal surface of proximal and distal convoluted tubules reacted positively. By electron microscopy the enzyme activity in the flask cells was localized mainly on the surface of microvilli like cytoplasmic processes which lined the lumen of intracellular secretion capillaries. The existence of carbonic anhydrase in the flask cells is related to a possible physiological function of these cells.
采用豪斯勒(1958年)和汉森(1967年)的碳酸钴法,在爪蟾中肾中间节段和集合小管的烧瓶状细胞中证实了碳酸酐酶活性。此外,近端和远端曲小管的管腔表面反应呈阳性。通过电子显微镜观察,烧瓶状细胞中的酶活性主要定位于微绒毛表面,这些微绒毛类似排列在细胞内分泌毛细血管管腔的细胞质突起。烧瓶状细胞中碳酸酐酶的存在与这些细胞可能的生理功能有关。