Jonas L
Acta Histochem. 1982;70(1):43-53.
An antiserum against carbonic anhydrase from bovine erythrocytes was produced and tested on paraffin sections of bovine rennet-bag and kidney with an indirect immunofluorescence technique. A specific immunofluorescence was found in the parietal cells, mucoid surface, and foveolar cells of rennet-bag mucosa. In the kidney, there was a positive reaction mainly in the distal nephron segments as the loops of Henle and the distal tubules and also in the collecting tubes. The glomeruli and proximal tubules reacted negatively. The specificity of the found distribution of immunofluorescence was confirmed by some control reactions and was also demonstrated by partial congruence with results obtained by the cobalt method. The demonstrated enzyme activities are compared with results from the literature.
制备了一种针对牛红细胞碳酸酐酶的抗血清，并采用间接免疫荧光技术在牛凝乳袋和肾脏的石蜡切片上进行了检测。在凝乳袋黏膜的壁细胞、黏液表面和小凹细胞中发现了特异性免疫荧光。在肾脏中，主要在远端肾单位节段（如髓袢和远端小管）以及集合管中有阳性反应。肾小球和近端小管反应为阴性。通过一些对照反应证实了所发现的免疫荧光分布的特异性，并且与钴法获得的结果部分一致也证明了这一点。将所显示的酶活性与文献结果进行了比较。
