Rieu-Lesme F, Fonty G, Doré J
Laboratoire de Microbiologie, INRA, Centre de Recherches de Clermont-Ferrand-Theix, Saint-Genès-Champanelle, France.
FEMS Microbiol Lett. 1995 Jan 1;125(1):77-82. doi: 10.1111/j.1574-6968.1995.tb07338.x.
A new H2/CO2-utilizing acetogenic bacterium was isolated from the rumen of a mature deer. This is the first report of a spore-forming Gram-negative bacterial species from the rumen. The organism was a strictly anaerobic, motile rod and was able to grow autotrophically on hydrogen and carbon dioxide. Acetate was the major product detected. Glucose, fructose and lactate were also fermented heterotrophically. The optimum pH for growth was 7.0-7.5, and the optimum temperature was 37-42 degrees C. Yeast extract was required for growth and rumen fluid was highly stimulatory. The DNA base ratio was 52.9 +/- 0.5 mol% G+C. On the basis of these characteristics and fermentation products, the isolate was considered to be different from acetogenic bacteria described previously.
从一只成年鹿的瘤胃中分离出一种新的利用H2/CO2的产乙酸细菌。这是首次报道来自瘤胃的形成芽孢的革兰氏阴性细菌物种。该微生物是严格厌氧、能动的杆菌，能够在氢气和二氧化碳上自养生长。检测到的主要产物是乙酸盐。葡萄糖、果糖和乳酸也能被异养发酵。生长的最适pH为7.0 - 7.5，最适温度为37 - 42摄氏度。生长需要酵母提取物，瘤胃液具有高度刺激作用。DNA碱基比为52.9 +/- 0.5 mol% G+C。基于这些特征和发酵产物，该分离物被认为与先前描述的产乙酸细菌不同。
