Loubiere P, Goma G, Lindley N D
Département de Génie Biochimique et Alimentaire, UA-CNRS-544, INSA-CTBM, Toulouse, France.
Antonie Van Leeuwenhoek. 1990 Feb;57(2):83-9. doi: 10.1007/BF00403159.
The inhibitory effects of organic acids produced as fermentation end-products during methylotrophic growth of the acidogenic anaerobe, Eubacterium limosum have been investigated. Precise quantification of the intracellular concentrations of acetate and butyrate, together with delta pH measurements indicate that butyrate efflux cannot be explained by a process of passive diffusion. Intracellular concentrations of butyrate were significantly lower than those of the culture broth. It is argued that growth inhibition by butyrate is due to energetic limitations resulting from the energy drain associated with this non-passive efflux mechanism.
对产酸厌氧菌黏液真杆菌在甲基营养生长过程中作为发酵终产物产生的有机酸的抑制作用进行了研究。对细胞内乙酸盐和丁酸盐浓度的精确量化，以及ΔpH测量表明，丁酸盐外流不能用被动扩散过程来解释。细胞内丁酸盐的浓度明显低于培养液中的浓度。有人认为，丁酸盐对生长的抑制是由于与这种非被动外流机制相关的能量消耗导致的能量限制。
