[1-磷酸葡萄糖和无机磷酸对糖原与肌肉磷酸化酶b结合的影响]
[Effect of glucose 1-phosphate and inorganic phosphate on binding of muscle phosphorylase b by glycogen].
作者信息
Chebotareva N A, Lisovskaia N P, Kurganov B I
出版信息
Biokhimiia. 1981 Jul;46(7):1221-5.
PMID:6791706
Abstract
Using analytical ultracentrifugation, the affinity of rabbit skeletal muscle phosphorylase b for glycogen was shown to increase 11.6-fold in the presence of saturating concentrations of glucose 1-phosphate and to decrease 3.7-fold in the presence of saturating concentrations of inorganic phosphate (20 degrees, 0.05 M glycyl-glycine buffer, pH 6.8, ionic strength 0.3).
摘要
通过分析超速离心法表明，在存在饱和浓度的1-磷酸葡萄糖时，兔骨骼肌磷酸化酶b对糖原的亲和力增加了11.6倍；而在存在饱和浓度的无机磷酸盐时，其亲和力降低了3.7倍（20摄氏度，0.05M甘氨酰-甘氨酸缓冲液，pH 6.8，离子强度0.3）。
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