Czuprynski C J, Balish E
Infect Immun. 1981 Aug;33(2):348-54. doi: 10.1128/iai.33.2.348-354.1981.
Serum from both germfree and conventional rats, but not plasma or plasma serum, killed Listeria monocytogenes in vitro by a calcium-dependent mechanism that was independent of either complement or lysozyme and was not inhibited by the addition of iron. The listericidin was purified by passing either rat serum or platelet lysate through a nitrocellulose filter (0.2 micrometer) and eluting the activity from the filter with 0.02 N HCl. The partially purified listericidin was heat stable (56 degrees C for 30 min), removed by absorption with zymosan or bentonite, sensitive to treatment with trypsin or pronase, and inhibited by the addition of citrate (0.045 M), suggesting that the serum listericidin is a cationic protein. The development of serum listericidal activity, which could be important in the innate resistance of rats to L. monocytogenes, was dependent on both age and microbial status. Although some discrepancies exist between the serum listericidin and previous descriptions of serum beta-lysin, we believe that the rat serum listericidin is a similar cationic protein.
无菌大鼠和普通大鼠的血清（而非血浆或血浆血清），通过一种依赖钙的机制在体外杀死单核细胞增生李斯特菌，该机制独立于补体或溶菌酶，且不受铁添加的抑制。通过将大鼠血清或血小板裂解物通过硝酸纤维素滤膜（0.2微米），并用0.02 N盐酸从滤膜上洗脱活性，来纯化李斯特杀菌素。部分纯化的李斯特杀菌素热稳定（56℃ 30分钟），可被酵母聚糖或膨润土吸附去除，对胰蛋白酶或链霉蛋白酶处理敏感，并被添加柠檬酸盐（0.045 M）抑制，这表明血清李斯特杀菌素是一种阳离子蛋白。血清李斯特杀菌活性的发展，这可能对大鼠对单核细胞增生李斯特菌的天然抵抗力很重要，取决于年龄和微生物状态。尽管血清李斯特杀菌素与先前对血清β-溶素的描述之间存在一些差异，但我们认为大鼠血清李斯特杀菌素是一种类似的阳离子蛋白。
