Hof H
Med Microbiol Immunol. 1984;173(4):207-18. doi: 10.1007/BF02122112.
Different strains of Listeria monocytogenes serovar 1/2a were used to infect mice. A smooth, haemolytic strain multiplied in the spleen of normal adult mice and induced a long-lasting immunity to subsequent challenge infection. When the animals were treated with dextran sulphate (DS) Listeriae multiplied more rapidly and death followed within a few days. When normal baby mice were infected with this organism, fatal infection occurred. In nude mice a chronic infection developed. Secondly, a rough, haemolytic strain was used to infect mice. In normal adult mice no multiplication of this strain was observed. This holds true also for DS-treated animals. In nude mice the bacteria were eliminated slowly. Normal baby mice could only be killed if the infective dose was increased. This strain was considered to be intermediate in virulence. The infection stimulated a considerable immune response in mice, although to a lesser degree than the smooth, haemolytic Listeria strain. Thirdly, a smooth, non-haemolytic strain of L. monocytogenes serovar 1/2a was used. The bacteria were rapidly eliminated in normal, in DS-treated and in nude animals. Normal baby mice did not develop fatal disease, so it was considered that this strain of L. monocytogenes serovar 1/2a was avirulent. This variant was found to be non-immunogenic. A boosting of immunity of animals by this avirulent Listeria strain was, however, found to be possible. A reasonable explanation for the rapid elimination of avirulent L. monocytogenes serovar 1/2a from mice cannot be presented at this time. Whereas it is known that the macrophage system and the T-lymphocytes play an essential role in the resistance to virulent Listeriae, there is no increased susceptibility of the avirulent bacteria to these defence mechanisms. Other bacterial properties, such as serum sensitivity and lysozyme susceptibility, are likewise considered to be unimportant.
使用不同菌株的单核细胞增生李斯特菌血清型1/2a感染小鼠。一种光滑、溶血的菌株在正常成年小鼠的脾脏中繁殖，并诱导对随后的攻击感染产生持久免疫力。当用硫酸葡聚糖（DS）处理动物时，李斯特菌繁殖得更快，几天后动物死亡。当正常幼鼠感染这种微生物时，会发生致命感染。在裸鼠中会发展为慢性感染。其次，使用一种粗糙、溶血的菌株感染小鼠。在正常成年小鼠中未观察到该菌株的繁殖。这对于用DS处理的动物也同样适用。在裸鼠中，细菌被缓慢清除。只有增加感染剂量才能杀死正常幼鼠。该菌株被认为毒力中等。这种感染在小鼠中刺激了相当大的免疫反应，尽管程度低于光滑、溶血的李斯特菌菌株。第三，使用单核细胞增生李斯特菌血清型1/2a的一种光滑、非溶血菌株。这种细菌在正常、用DS处理的动物和裸鼠中都被迅速清除。正常幼鼠没有发展成致命疾病，因此认为这种单核细胞增生李斯特菌血清型1/2a菌株无毒力。发现这种变体没有免疫原性。然而，发现用这种无毒力的李斯特菌菌株增强动物免疫力是可能的。目前无法对无毒力的单核细胞增生李斯特菌血清型1/2a从小鼠中迅速清除给出合理的解释。虽然已知巨噬细胞系统和T淋巴细胞在抵抗有毒力的李斯特菌中起重要作用，但无毒力细菌对这些防御机制的敏感性并没有增加。其他细菌特性，如血清敏感性和溶菌酶敏感性，同样被认为不重要。
