粘质沙雷氏菌“快速”和“延迟”人血清敏感菌株的进一步特性分析。

Further characterization of "promptly" and "delayed" human serum-sensitive strains of Serratia marcescens.

作者信息

Traub W H, Fukushima P I

出版信息

Zentralbl Bakteriol Orig A. 1979 Dec;245(4):495-511.

Abstract

The kinetics of the bactericidal activity of 80 vol% of fresh human serum against representative 'delayed serum-sensitive' (DSS) and 'promptly serum-sensitive' (PSS) strains of Serratia marcescens were further examined with regard to various chemical and absorption procedures known to affect various components of the alternative and classical pathways of human complement activation. Inulin treatment of fresh human serum failed to diminish serum bactericidal activity against DSS and PSS assay strains. Fresh human serum that had been depleted of properdin (factor P) through absorption with zymosan, was as active as control serum against DSS strains of S. marcescens; however, PSS strains were killed in a 'delayed' fashion. Human serum that had been heat-inactivated at 50 degrees C for minutes (depletion of factor B), no longer killed DSS strains, whereas PSS strains of S. marcescens and the PSS control strain Escherichia coli C were killed in a slightly delayed fashion. Hydrazine-hydrate treatment (inactivation of C3 of the complement system) and exposure of fresh human serum to dithiothreitol completely abolished serum bactericidal activity. Bentonite-absorbed fresh human serum no longer killed DSS strains of S. marcescens; some PSS strains of S. marcescens were killed in a delayed manner, whereas control strain E. coli C was as PSS as before. Addition of Seitz-filtered fresh human serum, that lacked beta-lysin and was deficient in lysozyme, to bentonite-absorbed human serum restored bactericidal activity against DSS and PSS strains of S. marcescens; addition to heat-inactivated or Seitz-filtered, heat-inactivated human serum failed to do so. Therefore, bentonite absorption removed to a heat-labile component from fresh human serum clearly different from beta-lysin and lysozyme. Furthermore, human serum beta-lysin and lysozyme were not required for serum-mediated killing of S. marcescens strains of either serum susceptibility category.

摘要

针对已知会影响人类补体激活替代途径和经典途径各成分的各种化学及吸收程序，进一步研究了80%体积分数的新鲜人血清对粘质沙雷氏菌代表性“迟发性血清敏感”（DSS）菌株和“即时血清敏感”（PSS）菌株的杀菌活性动力学。用菊粉处理新鲜人血清未能降低其对DSS和PSS检测菌株的杀菌活性。通过与酵母聚糖吸收而耗尽备解素（因子P）的新鲜人血清，对粘质沙雷氏菌DSS菌株的活性与对照血清相同；然而，PSS菌株是以“延迟”方式被杀死的。在50℃加热灭活数分钟的人血清（因子B耗尽），不再能杀死DSS菌株，而粘质沙雷氏菌的PSS菌株和PSS对照菌株大肠杆菌C是以稍延迟的方式被杀死的。水合肼处理（补体系统C3失活）以及将新鲜人血清暴露于二硫苏糖醇完全消除了血清杀菌活性。经皂土吸收的新鲜人血清不再能杀死粘质沙雷氏菌的DSS菌株；一些粘质沙雷氏菌的PSS菌株是以延迟方式被杀死的，而对照菌株大肠杆菌C与之前一样属于PSS类型。向经皂土吸收的人血清中添加经赛茨滤器过滤且缺乏β - 溶素并溶菌酶含量不足的新鲜人血清，可恢复对粘质沙雷氏菌DSS和PSS菌株的杀菌活性；添加到热灭活或经赛茨滤器过滤并热灭活的人血清中则无法恢复。因此，皂土吸收去除了新鲜人血清中一种明显不同于β - 溶素和溶菌酶的热不稳定成分。此外，血清介导杀死任何一种血清敏感性类别的粘质沙雷氏菌菌株都不需要人血清β - 溶素和溶菌酶。