Carlsson H E, Sundblad G, Hammarström S, Lönngren J
Carbohydr Res. 1978 Jul;64:181-8. doi: 10.1016/s0008-6215(00)83699-x.
Glycoproteins derived from intestinal mucus were isolated by phenol-water extraction of feces from germ-free rats. The water-soluble glycoproteins were subjected to alkaline-borohydride degradation, and three different oligosaccharide alditols were isolated. The structures of thse were determined by using methylation analysis and n.m.r. spectroscopy as the principal methods. One disaccharide alditol and one trisaccharide alditol were characterised as beta-D-GlcNAcp-(1 leads to 3)-D-GalNAcol and alpha-L-Fucp-(1 leads to 2)-beta-D-Galp-(1 leads to 3)-D-GalNAcol. The third oligosaccharide alditol was a blood-group A-active tetrasaccharide alditol for which the structure alpha-D-GalNAcp-(1 leads to 3)-[alpha-L-Fucp-(1 leads to 2)]-beta-D-Galp-(1 leads to 3)-D-GalNAcol is proposed.
通过对无菌大鼠粪便进行苯酚-水萃取，分离出了源自肠道黏液的糖蛋白。将水溶性糖蛋白进行碱性硼氢化物降解，分离出了三种不同的低聚糖糖醇。主要通过甲基化分析和核磁共振光谱法确定了它们的结构。一种二糖糖醇和一种三糖糖醇被鉴定为β-D-葡萄糖胺基-（1→3）-D-半乳糖胺醇和α-L-岩藻糖基-（1→2）-β-D-半乳糖基-（1→3）-D-半乳糖胺醇。第三种低聚糖糖醇是一种具有血型A活性的四糖糖醇，其结构被推测为α-D-半乳糖胺基-（1→3）-[α-L-岩藻糖基-（1→2）]-β-D-半乳糖基-（1→3）-D-半乳糖胺醇。
