一种快速分离人IgM骨髓瘤蛋白的方法。
A rapid procedure for the isolation of human IgM myeloma proteins.
作者信息
Jehanli A, Hough D
出版信息
J Immunol Methods. 1981;44(2):199-204. doi: 10.1016/0022-1759(81)90347-1.
A two-step rapid procedure for the isolation of human IgM paraproteins from plasma samples is described. It involves gel filtration on Ultrogel AcA 34 followed by anion exchange chromatography on DEAE-Sepharose CL6B. The overall yield of IgM is greater than 70% and the final product is pure as judged by immunoelectrophoresis and SDS-polyacrylamide gel electrophoresis. The procedure is applicable to both euglobulin and cryoglobulin IgM proteins of a wide range of electrophoretic mobilities.
本文描述了一种从血浆样本中分离人IgM副蛋白的两步快速方法。该方法包括在Ultrogel AcA 34上进行凝胶过滤，然后在DEAE-Sepharose CL6B上进行阴离子交换色谱。IgM的总产率大于70%，通过免疫电泳和SDS-聚丙烯酰胺凝胶电泳判断，最终产物是纯的。该方法适用于各种电泳迁移率的优球蛋白和冷球蛋白IgM蛋白。
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