Ide F, Ishikawa T, Takayama S, Umemura S
J Oral Pathol. 1981 Apr;10(2):113-23. doi: 10.1111/j.1600-0714.1981.tb01256.x.
A system in which oral tissues of inbred F344 adult rats and Syrian golden hamster embryos were used in combination with autoradiography was developed for measurement of unscheduled DNA synthesis (UDS). For this, oral mucosa, submandibular gland, tooth germ and mandible in short-term organ cultures were treated with 4-nitroquinoline 1-oxide or N-methyl-N-nitrosourea plus (methyl-3H)thymidine. Significant numbers of silver grains, indicating UDS, were detected over the nuclei of cells of all these tissues except rat salivary gland after treatment with carcinogens. This autoradiographic method is suitable for detection of UDS in oral tissues in conditions mimicking those in vivo. Results obtained in this study indicated a potential use of this system for studies on the mechanism of carcinogenesis at a cellular level comparable to in vivo carcinogenesis studies on oral tissues.
开发了一种系统，该系统将近交F344成年大鼠的口腔组织和叙利亚金黄地鼠胚胎与放射自显影相结合，用于测量非预定DNA合成（UDS）。为此，将短期器官培养中的口腔黏膜、下颌下腺、牙胚和下颌骨用4-硝基喹啉1-氧化物或N-甲基-N-亚硝基脲加（甲基-³H）胸腺嘧啶处理。在用致癌物处理后，除大鼠唾液腺外，所有这些组织的细胞核上均检测到大量表明UDS的银颗粒。这种放射自显影方法适用于在模拟体内条件下检测口腔组织中的UDS。本研究获得的结果表明，该系统在细胞水平上研究致癌机制方面具有潜在用途，可与口腔组织的体内致癌研究相媲美。
