Cowpe J G, Longmore R B
J Oral Pathol. 1981 Apr;10(2):81-6. doi: 10.1111/j.1600-0714.1981.tb01252.x.
Malignant cells usually have an abnormal DNA content demonstrable by cytophotometry of Feulgen stained tissue. The diagnostic reliability of the technique is reduced because some malignant cells have a normal Feulgen DNA content. If Feulgen DNA content can be combined with measurements of additional cytological characteristics known to vary in malignancy, reliable diagnostic criteria may be forthcoming. In this study, Feulgen DNA content and nuclear area were estimated in 50 squames from the buccal mucosa in each of 14 young adults. Nuclear Feulgen DNA content was determined using an M85 microdensitometer and nuclear area was measured using a Reichert MOP image analyser. In each specimen, Feulgen DNA content was consistent with a nonreplicating cell population. Although nuclear area varied in each specimen, the variation in mean nuclear area among the sample of 14 was not statistically significant. It is concluded that nuclear area provides an accurate baseline against which future measurements on abnormal tissue can be compared.
恶性细胞通常具有异常的DNA含量，这可通过对福尔根染色组织进行细胞光度测定来证明。由于一些恶性细胞具有正常的福尔根DNA含量，该技术的诊断可靠性降低。如果福尔根DNA含量能够与已知在恶性肿瘤中会发生变化的其他细胞学特征的测量相结合，那么可靠的诊断标准可能会出现。在本研究中，对14名年轻成年人每人颊黏膜的50个鳞状细胞进行了福尔根DNA含量和核面积的估计。使用M85微量密度计测定细胞核福尔根DNA含量，使用莱卡MOP图像分析仪测量核面积。在每个标本中，福尔根DNA含量与非复制细胞群体一致。尽管每个标本中的核面积有所不同，但14个样本的平均核面积变化在统计学上并不显著。结论是，核面积提供了一个准确的基线，可用于与未来对异常组织的测量进行比较。
