人肾癌组织中的芳基酰胺酶与正常肾和胎盘酶的比较。

Arylamidase from human renal cancer tissue in comparison with normal kidney and placental enzymes.

作者信息

Yamamoto H, Higashino K, Hada T, Sumikawa K, Yamamura Y

出版信息

Oncodev Biol Med. 1980;1(3):169-80.

Abstract

Arylamidase (E.C. 3.4.11.2) was solubilized from renal cancer tissues by bromelain treatment, and its properties were compared with those of normal kidney and placental enzymes after partial purification. Their column chromatograms on TEAE-cellulose revealed a slight, but constant difference in the negative charge, namely, in normal kidney, renal cancer tissue, and placental enzymes in increasing order. An electrophoretic study on polyacrylamide gel showed comparable results. On the other hand, when treated with neuraminidase prior to electrophoresis, the renal cancer tissue and kidney enzymes came to have an identical mobility, while the placental enzyme still had a faster mobility than the others. The renal cancer tissue arylamidase was not clearly distinguished from the kidney and placental enzymes with respect to molecular weight, Michaelis constant, pH optimum, heat stability, behavior to divalent cations or chelating agents, susceptibility to urea or amino acids, inhibition by sulfhydryl agents, and immunological properties. These results suggest that renal cancer tissue and kidney enzymes are similar glycoproteins, simply different in sialic acid content, and that these two enzymes are different from the placental enzyme in the structure of the peptide portion and/or carbohydrate portions other than sialic acid residues.

摘要

通过菠萝蛋白酶处理从肾癌组织中溶解出芳基酰胺酶（E.C. 3.4.11.2），并在部分纯化后将其性质与正常肾脏和胎盘酶的性质进行比较。它们在TEAE - 纤维素上的柱色谱图显示出负电荷存在轻微但恒定的差异，即正常肾脏、肾癌组织和胎盘酶中的负电荷按递增顺序排列。在聚丙烯酰胺凝胶上进行的电泳研究显示了类似的结果。另一方面，在电泳前用神经氨酸酶处理时，肾癌组织和肾脏酶的迁移率变得相同，而胎盘酶的迁移率仍然比其他酶快。就分子量、米氏常数、最适pH值、热稳定性、对二价阳离子或螯合剂的反应、对尿素或氨基酸的敏感性、巯基试剂的抑制作用以及免疫特性而言，肾癌组织芳基酰胺酶与肾脏和胎盘酶没有明显区别。这些结果表明，肾癌组织和肾脏酶是相似的糖蛋白，只是唾液酸含量不同，并且这两种酶在肽部分和/或除唾液酸残基以外的碳水化合物部分的结构上与胎盘酶不同。