Sato K, Sato F
Am J Physiol. 1982 May;242(5):C353-9. doi: 10.1152/ajpcell.1982.242.5.C353.
Sr2+ substitutes for Ca2+ in methacholine (MCH)-evoked sweat secretion in isolated simian eccrine sweat glands, but four to eight times greater concentrations of Sr2+ were required to achieve the same effect as did a given Ca2+ concentration. In Ca2+-free Sr2+ medium the anticholinergic effect of atropine was reduced by about four orders of magnitude. In the Sr2+ medium, spontaneous sweat secretion (SSS) was also observed in the absence of any pharmacological stimuli. SSS occurs slowly, reaching a plateau after 30 min incubation. The maximal SSS is a saturation function of Sr2+ concentration reaching a maximum at 8 mM. Sr2+-induced SSS was inhibitable by removal of Sr2+, low bath temperature (10-14 degrees C), ouabain (5 X 10(-6) M), and D 600 (10(-3) M). Ca2+ was found to inhibit Sr2+-induced SSS, but a Schild plot for the Ca2+ and Sr2+ dose relationship failed to show a slope of unity. The data suggest that Sr2+-induced SSS represents actual secretory processes, which are triggered by Sr2+ leaking into the cell through as yet undefined membrane channels.
在离体的猿猴外泌汗腺中，Sr2+可替代Ca2+参与乙酰甲胆碱（MCH）诱发的汗液分泌，但要达到与给定Ca2+浓度相同的效果，所需的Sr2+浓度要高4至8倍。在无Ca2+的Sr2+培养基中，阿托品的抗胆碱能作用降低了约4个数量级。在Sr2+培养基中，在没有任何药理学刺激的情况下也观察到了自发性汗液分泌（SSS）。SSS出现缓慢，孵育30分钟后达到平稳状态。最大SSS是Sr2+浓度的饱和函数，在8 mM时达到最大值。去除Sr2+、降低浴温（10 - 14摄氏度）、使用哇巴因（5×10(-6) M）和D 600（10(-3) M）可抑制Sr2+诱导的SSS。发现Ca2+可抑制Sr2+诱导的SSS，但Ca2+与Sr2+剂量关系的施尔德图未显示出斜率为1。数据表明，Sr2+诱导的SSS代表实际的分泌过程，这是由Sr2+通过尚未明确的膜通道泄漏到细胞中触发的。
