Richardson C L, Verna J, Schulman G E, Shipp K, Grant A D
Environ Mutagen. 1981;3(5):545-53. doi: 10.1002/em.2860030506.
Several metals are known mutagens and carcinogens. These metals effectively displace acridine orange from DNA when measured by fluorescence polarization. Displacement of 50% of the acridine orange is obtained with less than 0.5 mM concentrations of lead, manganese, cobalt, zinc, cadmium, nickel, iron, copper, and cis-platinum. In contrast, greater than 80 mM concentrations of lithium, sodium, and potassium are required to displace an equivalent amount of acridine orange from calf thymus DNA. Although cis-platinum shows the best DNA reactivity in this assay, the interaction between this metal and DNA does not occur immediately, as it does for the other metals tested. These results indicate the acridine orange displacement assay provides a relative measure of the interaction of metals with DNA, and this DNA reactivity shows a positive correlation with mutagenic/carcinogenic potential.
有几种金属是已知的诱变剂和致癌物。通过荧光偏振测量时，这些金属能有效地将吖啶橙从DNA中置换出来。铅、锰、钴、锌、镉、镍、铁、铜和顺铂的浓度低于0.5 mM时就能置换50%的吖啶橙。相比之下，需要大于80 mM的锂、钠和钾浓度才能从小牛胸腺DNA中置换等量的吖啶橙。尽管顺铂在该测定中显示出最佳的DNA反应性，但这种金属与DNA之间的相互作用不像其他测试金属那样立即发生。这些结果表明，吖啶橙置换测定法提供了金属与DNA相互作用的相对测量值，并且这种DNA反应性与诱变/致癌潜力呈正相关。
