Hosoi K, Kida T, Ueha T
J Biochem. 1981 Jun;89(6):1793-8. doi: 10.1093/oxfordjournals.jbchem.a133379.
Trypsin-like and chymotrypsin-like esteroprotease isozymes of the mouse submandibular gland were separated by isoelectric focusing. In normal female mice the following pI-isozyme activities were found; pI-4.6, -5.6 (shoulder), -5.8, -7.1, and -9.9, hydrolytic activities for benzoylarginine ethylester (BAEE) (trypsin-like enzymes), and pI-4.7 and -10.3 hydrolytic activities for acetyltyrosine ethylester (ATEE) (chymotrypsin-like enzymes). In mice with testicular feminization (Tfm mice), only pI4.6 hydrolytic activity for BAEE was found; no ATEE hydrolytic activity was detected. In normal female mice, both 5 alpha-dihydrotestosterone (5 alpha-DHT) and tri-iodo-L-thyronine (T3) significantly increased all these isozymes except the pI-4.6 hydrolytic activity for BAEE. In Tfm mice, T3 also increased all these isozymes except the pI-4.6 hydrolytic activity for BAEE, but 5 alpha-DHT had no effect on any enzymes. These results suggest that the pI-4.6 hydrolytic activity for BAEE is non-inducible by the two hormones. Androgen does not seem to be involved in the inductions of these esteroproteases by T3.
通过等电聚焦分离了小鼠颌下腺的胰蛋白酶样和糜蛋白酶样酯蛋白酶同工酶。在正常雌性小鼠中发现了以下pI同工酶活性：pI-4.6、-5.6（肩峰）、-5.8、-7.1和-9.9，对苯甲酰精氨酸乙酯（BAEE）的水解活性（胰蛋白酶样酶），以及pI-4.7和-10.3对乙酰酪氨酸乙酯（ATEE）的水解活性（糜蛋白酶样酶）。在睾丸雌性化小鼠（Tfm小鼠）中，仅发现了对BAEE的pI4.6水解活性；未检测到ATEE水解活性。在正常雌性小鼠中，5α-二氢睾酮（5α-DHT）和三碘-L-甲状腺原氨酸（T3）均显著增加了所有这些同工酶，但对BAEE的pI-4.6水解活性除外。在Tfm小鼠中，T3也增加了所有这些同工酶，但对BAEE的pI-4.6水解活性除外，而5α-DHT对任何酶均无影响。这些结果表明，对BAEE的pI-4.6水解活性不受这两种激素的诱导。雄激素似乎不参与T3对这些酯蛋白酶的诱导作用。
