Castro-Vazquez A, Ojeda S R, Krulich L, McCann S M
Neuroendocrinology. 1981;33(4):193-8. doi: 10.1159/000123228.
Serum TSH, LH and FSH were measured at various times during the day, during the estrous cycle of the rat. Proestrous surges of FSH and LH were detected as previously reported. TSH values fell to low levels in the late afternoon on each day of the cycle. There was a significant elevation of TSH at 13.00 h on both proestrus and estrus. The elevation of TSH on proestrus was accompanied by a significant fall in pituitary TSH stores. LH stores were also significantly depleted by 19.00 h on proestrus. Cervicovaginal stimulation by a glass rod provoked a significant rise in serum TSH 3 h after stimulation at 10.00 h on estrus but not on other days of the cycle. Responsiveness to a test dose of TRH varied during the cycle. It was maximal on proestrus and declined to minimum values on diestrus day II. Values were further lowered after ovariectomy but were restored to diestrus day II levels by treatment with estradiol. It is concluded that there are important cyclic variations in TSH release in the female rat, that cervicovaginal stimulation can provoke TSH release on estrus and that these changes are associated with altered responsiveness to TRH. We conclude that ovarian steroids modulate the release of TSH from the pituitary and affect the responsiveness to cervicovaginal stimulation. These changes are presumably brought about by alterations in TRH release and in responsiveness of the pituitary to the neurohormone.
在大鼠动情周期的不同时间测量血清促甲状腺激素（TSH）、促黄体生成素（LH）和促卵泡生成素（FSH）。如先前报道，检测到动情前期FSH和LH的高峰。在动情周期的每一天，TSH值在傍晚时降至低水平。在动情前期和动情期的13:00时，TSH有显著升高。动情前期TSH的升高伴随着垂体TSH储备的显著下降。在动情前期的19:00时，LH储备也显著减少。在动情期的10:00时，用玻璃棒刺激宫颈阴道，刺激后3小时血清TSH显著升高，而在动情周期的其他日子则无此现象。对促甲状腺激素释放激素（TRH）试验剂量的反应性在动情周期中有所变化。在动情前期最大，在动情后期第II天降至最小值。卵巢切除术后数值进一步降低，但用雌二醇治疗后恢复到动情后期第II天的水平。得出的结论是，雌性大鼠TSH释放存在重要的周期性变化，宫颈阴道刺激可在动情期激发TSH释放，且这些变化与对TRH反应性的改变有关。我们得出结论，卵巢类固醇调节垂体TSH的释放，并影响对宫颈阴道刺激的反应性。这些变化可能是由TRH释放的改变以及垂体对该神经激素反应性的改变引起的。
