人体血液p50与2,3-二磷酸甘油酸、二氧化碳和氢离子关系的方程式及列线图

Equations and nomogram for the relationship of human blood p50 to 2,3-diphosphoglycerate, CO2, and H+.

作者信息

Samaja M, Mosca A, Luzzana M, Rossi-Bernardi L, Winslow R M

出版信息

Clin Chem. 1981 Nov;27(11):1856-61.

Abstract

We describe a new method for tonometry of small amounts of blood (up to 0.25 mL) at known pO2, PCO2, and temperature, in small, reusable, closed Pyrex flasks. Equilibrated blood is analyzed for oxygen saturation, pH, and organic phosphate concentration with standard techniques, and its p50 (the pO2 at which hemoglobin is half-saturated with oxygen) is determined with full control of all the variables known to affect it. The SD in the measurement of p50 is 0.044 kPa (0.33 mmHg). We made 63 determinations of p50 on normal human blood under different conditions of pH and pCO2, and with different concentrations of 2,3-diphosphoglycerate and ATP. Empirical equations and a nomogram were derived, which allow the calculation of p50 from known values of pCO2, pH, and [2,3-DPG]/[Hb4] molar ratio with a SD of 97 and 114 Pa (0.73 and 0.86 mmHg), respectively.

摘要

我们描述了一种新方法，可在已知的氧分压（pO₂）、二氧化碳分压（PCO₂）和温度条件下，对小量血液（最多0.25 mL）进行眼压测量，测量在小型、可重复使用的密闭派热克斯玻璃烧瓶中进行。用标准技术分析平衡后的血液中的氧饱和度、pH值和有机磷酸盐浓度，并在完全控制所有已知影响因素的情况下测定其p50（血红蛋白氧饱和度为50%时的氧分压）。p50测量的标准差为0.044千帕（0.33毫米汞柱）。我们在不同pH值和PCO₂条件下，以及不同浓度的2,3 - 二磷酸甘油酸和三磷酸腺苷情况下，对正常人血液进行了63次p50测定。得出了经验方程和列线图，可根据已知的PCO₂、pH值和[2,3 - DPG]/[Hb₄]摩尔比计算p50，标准差分别为97和114帕（0.73和0.86毫米汞柱）。