Chan K M, Ladenson J H
Clin Chem. 1981 Nov;27(11):1896-8.
Total protein concentration in the serum of a patient with hyperviscosity syndrome differed as measured by the biuret procedure in the DuPont aca (80 g/L) and the SMA 12/60 (105 g/L), owing to viscosity-dependent errors with the aca sampling system; the magnitude depended on sample temperature and volume of sample aspirated. This kind of error was not observed with the SMA 12/60 and was far less severe when a Micromedic sampler-dilutor was tested. It could be eliminated in the case of the aca by adding sample to test packs with a syringe rather than with the aca automated sampler-dilutor. We thus recommend use of the syringe method when unusually viscous samples (serum or other body fluids) are analyzed in the aca.
采用双缩脲法在杜邦aca仪器（80 g/L）和SMA 12/60仪器（105 g/L）上测定高黏滞综合征患者血清中的总蛋白浓度时，结果存在差异，这是由于aca采样系统存在黏度依赖性误差；误差大小取决于样品温度和吸取的样品体积。在SMA 12/60仪器上未观察到此类误差，在测试微量医学采样稀释器时此类误差也小得多。对于aca仪器，通过用注射器将样品添加到测试包中而非使用aca自动采样稀释器，可消除此类误差。因此，我们建议在aca仪器上分析异常黏稠的样品（血清或其他体液）时采用注射器法。
