Powers J M, Schlaepfer W W, Willingham M C, Hall B J
J Neuropathol Exp Neurol. 1981 Nov;40(6):592-612. doi: 10.1097/00005072-198111000-00002.
Samples of human cerebral cortex were obtained from twelve autopsied patients with Alzheimer's disease or "normal" aging. Rabbit or goat anti-human antisera to the following plasma proteins: IgG, F(ab')2, Fc, kappa and lambda light chains, IgM, IgA, fibrinogen, albumin, C3, lysozyme, haptoglobin, macroglobulin, and microglobulin; antibodies to the following intracellular proteins: glial fibrillary acidic (GFA) protein, filamin, actin, non-muscle myosin, tubulin, cholinergic vesicle proteins, and neurofilament (NF) proteins were utilized in the immunoglobulin peroxidase bridge. Amyloid cores of classical or perivascular plaques and dyshoric angiopathy exhibited a strong reaction for intact IgG and for both of its light chains, moderate reactions for lysozyme, fibrinogen, albumin and IgA, and weak reactions for IgM, C3, Fc, F(ab')2, haptoglobin, macroglobulin and microglobulin. Antibodies to all three NF proteins, individually and pooled, stained dyshoric and plaque amyloid, while antibodies to other intracellular proteins did not. The coronae of classical plaques and many primitive plaques stained for GFA, but inconsistently for IgG, both light chains, lysozyme, actin, tubulin, and NF proteins. Affected vessels of three patients with Congophilic angiopathy were reactive for all plasma proteins (especially IgG, fibrinogen, and albumin) and for NF proteins. NF staining in Congophilic blood vessels, although variable, revealed a peripheral or adventitial distribution, whereas plasma proteins tended to be localized in the media of the vessel wall. The distributions of Congo red and NF positivity were often identical. Both NF and Congo red staining was sensitive to oxidation. Isolated NF proteins were Congophilic and capable of displaying apple-green birefringence. A hypothesis concerning the role of NF proteins in senile cerebral amyloid is presented.
取自12例患阿尔茨海默病或“正常”衰老的尸检患者的人类大脑皮质样本。在免疫球蛋白过氧化物酶桥中使用了兔或山羊抗人抗血清，以检测以下血浆蛋白：IgG、F(ab')2、Fc、κ和λ轻链、IgM、IgA、纤维蛋白原、白蛋白、C3、溶菌酶、触珠蛋白、巨球蛋白和微球蛋白；以及针对以下细胞内蛋白的抗体：胶质纤维酸性（GFA）蛋白、细丝蛋白、肌动蛋白、非肌肉肌球蛋白、微管蛋白、胆碱能囊泡蛋白和神经丝（NF）蛋白。经典或血管周围斑块及血管壁营养不良性血管病的淀粉样核心对完整IgG及其两条轻链均呈现强反应，对溶菌酶、纤维蛋白原、白蛋白和IgA呈现中度反应，对IgM、C3、Fc、F(ab')2、触珠蛋白、巨球蛋白和微球蛋白呈现弱反应。针对所有三种NF蛋白单独及混合使用时，均能使血管壁营养不良性和斑块淀粉样物质染色，而针对其他细胞内蛋白的抗体则不能。经典斑块的晕圈和许多原始斑块对GFA染色，但对IgG、两条轻链、溶菌酶、肌动蛋白、微管蛋白和NF蛋白的染色不一致。3例嗜刚果红血管病患者的受累血管对所有血浆蛋白（尤其是IgG、纤维蛋白原和白蛋白）及NF蛋白均有反应。嗜刚果红血管中的NF染色虽有变化，但显示为外周或外膜分布，而血浆蛋白倾向于定位在血管壁的中膜。刚果红和NF阳性的分布通常相同。NF和刚果红染色均对氧化敏感。分离出的NF蛋白具有嗜刚果红性并能呈现苹果绿双折射。本文提出了一个关于NF蛋白在老年脑淀粉样变中作用的假说。
