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[亚甲蓝在溶液中以及固定于大肠杆菌和枯草芽孢杆菌上的比较光动力效应]

[Comparative photodynamic effect of methylene blue in solution and immobilized on Escherichia coli and Bacillus subtilis].

作者信息

Savino A, Angeli G, Poletti A, Murgia S M

出版信息

Boll Soc Ital Biol Sper. 1981 Aug 15;57(15):1601-7.

PMID:6796100
Abstract

The photodynamic activity of Methylene Bleu (MB) in homogeneous aqueous solution and in heterogeneous phase was compared in order to experiment the disinfection of waters and waste waters using the photosensitation technique. Insolubilized MB was obtained by covalent bonding on a styrene - 2% Divinylbenzene copolymer. E. coli and B. subtilis were used as gram-positive and gram-negative test microorganism. In the present experimental condition high inactivation towards both microorganism was obtained without substantial difference between homogeneous and heterogeneous phases. Therefore the photooxidation with immobilized BM seem to exalte the potential advantage of this technique in water disinfection for the remarkable bactericidal activity associated with the oossibility of the reuse of the photosensitizer.

摘要

为了利用光敏化技术对水和废水进行消毒实验,比较了亚甲基蓝(MB)在均相水溶液和非均相中的光动力活性。通过在苯乙烯-2%二乙烯基苯共聚物上共价键合获得不溶性MB。使用大肠杆菌和枯草芽孢杆菌作为革兰氏阳性和革兰氏阴性测试微生物。在当前实验条件下,对两种微生物都有很高的灭活率,均相和非均相之间没有实质性差异。因此,固定化BM的光氧化似乎提升了该技术在水消毒中的潜在优势,因为其具有与光敏剂再利用可能性相关的显著杀菌活性。

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