Holandino C, Capella M A, Angluster J, Silva-Filho F C, Menezes S, Alviano C S
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
Indian J Biochem Biophys. 1998 Oct;35(5):284-90.
Cell surface properties, including hydrophobicity, zeta potential, carbohydrate and fatty acid components, were altered on treatment of E. coli K12 with methylene blue (MB) and direct electric current (DC). The treatment of fimbriated E. coli cells with MB greatly increased the agglutination of yeast cells when compared to untreated bacteria. However, this increased agglutination was markedly reduced when the bacteria were treated with MB plus DC. These results suggest that MB modifies cell surface components in the absence of light and these alterations are more pronounced when cells are treated simultaneously with MB and DC.
用亚甲蓝（MB）和直流电（DC）处理大肠杆菌K12后，其细胞表面特性发生了改变，包括疏水性、zeta电位、碳水化合物和脂肪酸成分。与未处理的细菌相比，用MB处理有菌毛的大肠杆菌细胞大大增加了酵母细胞的凝集。然而，当细菌用MB加DC处理时，这种增加的凝集明显减少。这些结果表明，MB在无光条件下修饰细胞表面成分，并且当细胞同时用MB和DC处理时，这些改变更为明显。
