Williams G M, Laspia M F, Mori H, Hirono I
Cancer Lett. 1981 May;12(4):329-33. doi: 10.1016/0304-3835(81)90175-0.
The genotoxicity of cycasin was examined in the standard hepatocyte primary culture (HPC)/DNA repair test and in the test supplemented with beta-glucosidase. Generally, no DNA repair was elicited by cycasin in the standard test except for one assay which showed a strong response. With the addition of beta-glucosidase to the test medium, cycasin elicited DNA repair with clear dependence on both dose and amount of beta-glucosidase. These results indicate that supplementation of the HPC/DNA repair test with the appropriate should be useful in detecting potentially genotoxic glucosides and suggests that supplementation with other specific enzymes could compensate for extrahepatic biotransformation processes required prior to final activation by hepatocytes.
在标准肝细胞原代培养（HPC）/DNA修复试验以及添加β-葡萄糖苷酶的试验中检测了苏铁素的遗传毒性。一般来说，在标准试验中，除了一项显示出强烈反应的测定外，苏铁素不会引发DNA修复。在试验培养基中添加β-葡萄糖苷酶后，苏铁素引发了DNA修复，且明显依赖于β-葡萄糖苷酶的剂量和量。这些结果表明，在HPC/DNA修复试验中补充适当的物质有助于检测潜在的遗传毒性糖苷，并表明补充其他特定酶可以弥补肝细胞最终激活之前所需的肝外生物转化过程。
