内灌注小龙虾节段轴突中的电紧张性耦合
Electrotonic coupling in internally perfused crayfish segmented axons.
作者信息
Johnston M F, Ramón F
出版信息
J Physiol. 1981 Aug;317:509-18. doi: 10.1113/jphysiol.1981.sp013840.
Abstract
We have developed a technique for cannulation and internal perfusion of crayfish segmented lateral axons. Experiments on perfused and non-perfused axons lead to the following conclusions: 1. Internally perfused segmented axons behave very similarly to non-perfused axons. 2. The axial electrical resistance of the junctional region is almost as low as a comparable segment of axon. 3. Neither intracellular Ca2+ nor H+ is effective in disrupting the intercellular communication pathway in perfused axons. On the basis of these findings we have formulated a hypothesis for cellular control of intercellular coupling based on the existence of a soluble intermediate for Ca2+ or H+-induced uncoupling. This hypothesis is consistent with data from both internally perfused and non-perfused axons.
摘要
我们已经开发出一种用于小龙虾节段性侧轴突插管和内部灌注的技术。对灌注轴突和未灌注轴突进行的实验得出以下结论：1. 内部灌注的节段性轴突的行为与未灌注轴突非常相似。2. 连接区域的轴向电阻几乎与轴突的可比节段一样低。3. 细胞内的Ca2+和H+均不能有效破坏灌注轴突中的细胞间通讯途径。基于这些发现，我们基于Ca2+或H+诱导解偶联的可溶性中间体的存在，提出了一种细胞间偶联的细胞控制假说。该假说与内部灌注轴突和未灌注轴突的数据均一致。
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