Carne H R, Onon E O
J Hyg (Lond). 1982 Apr;88(2):173-91. doi: 10.1017/s0022172400070054.
The exotoxins produced by ten strains of C. ulcerans (two human, six bovine and two equine) have been studied. On the criteria of toxin-antitoxin neutralisation and immunoprecipitation tests using highly specific diphtheria and C. ovis antitoxins with crude toxic filtrates, (NH4)2SO4 concentrates, and partially purified chromatographic preparations of these, together with the presence or absence of inhibition of the action of staphylococcal beta-haemolysin, and the reaction produced when injected intradermally into rabbits, two toxins could be identified, namely diphtheria toxin and C. ovis toxin. There was no evidence for the production of a third toxin specific for C. ulcerans. Five strains produced both diphtheria and C. ovis toxins. In four diphtheria toxin predominated, but in the fifth C. ovis toxin predominated. Two strains produced only diphtheria toxin and two only C. ovis toxin, though there was good but not complete evidence that a third strain (Revell) also fell into this latter group. Considerable variation occurred in the concentration of each toxin and, where both were present, in the proportion of each.
对10株溃疡棒状杆菌（2株来自人类、6株来自牛、2株来自马）产生的外毒素进行了研究。以毒素 - 抗毒素中和以及免疫沉淀试验为标准，使用高特异性的白喉抗毒素和羊溃疡棒状杆菌抗毒素，分别作用于粗毒滤液、硫酸铵浓缩物及其部分纯化的色谱制剂，同时观察是否存在对葡萄球菌β - 溶血素作用的抑制以及皮内注射到兔子体内产生的反应，结果鉴定出两种毒素，即白喉毒素和羊溃疡棒状杆菌毒素。没有证据表明溃疡棒状杆菌会产生第三种特异性毒素。5株菌株同时产生白喉毒素和羊溃疡棒状杆菌毒素。在4株中白喉毒素占主导，但在第5株中羊溃疡棒状杆菌毒素占主导。2株仅产生白喉毒素，2株仅产生羊溃疡棒状杆菌毒素，不过有充分但不完全的证据表明第三株菌株（Revell）也属于后一组。每种毒素的浓度以及（两种毒素都存在时）它们各自的比例都存在相当大的差异。
