细胞色素P-450meg的底物特异性和诱导性研究。
Studies on the substrate specificity and inducibility of cytochrome P-450meg.
作者信息
Berg A, Rafter J J
出版信息
Biochem J. 1981 Jun 15;196(3):781-6. doi: 10.1042/bj1960781.
The cytochrome P-450-dependent steroid 15 beta-hydroxylase system in Bacillus megaterium A.T.C.C. 13368 was investigated with regard to its appearance in the cell with respect to the growth curve of the organism, with regard to its inducibility by a number of agents (among them some of the classical inducers of the mammalian liver microsomal cytochrome P-450 system) and with regard to its capacity to convert non-steroidal substances into oxygenated compounds. The enzyme was found to reach a maximum concentration in the cell during the stationary phase of the growth curve. Of all the agents tested as inducers, none showed any capacity to induce cytochrome P-450meg. Finally, of the substances tested as substrates only aniline (p-hydroxylation) was metabolized by the microbial enzyme system. This conversion might be related to the general oxygenase activity of haemoproteins. It is concluded that the substrate specificity of the B. megaterium hydroxylase system is narrow.
对巨大芽孢杆菌A.T.C.C. 13368中细胞色素P - 450依赖的甾体15β - 羟化酶系统进行了研究，内容包括该系统在细胞中的出现情况与生物体生长曲线的关系、多种试剂（包括一些哺乳动物肝脏微粒体细胞色素P - 450系统的经典诱导剂）对其的诱导能力以及将非甾体物质转化为氧化化合物的能力。发现该酶在生长曲线的稳定期在细胞中达到最高浓度。在所有作为诱导剂测试的试剂中，没有一种显示出诱导细胞色素P - 450meg的能力。最后，在作为底物测试的物质中，只有苯胺（对羟基化）被微生物酶系统代谢。这种转化可能与血红素蛋白的一般加氧酶活性有关。得出结论，巨大芽孢杆菌羟化酶系统的底物特异性较窄。
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