Fulco A J, Kim B H, Matson R S, Narhi L O, Ruettinger R T
Mol Cell Biochem. 1983;53-54(1-2):155-61. doi: 10.1007/BF00225251.
A soluble, cytochrome P-450-dependent fatty acid hydroxylase--epoxidase complex from Bacillus megaterium ATCC 14581 can be induced more than 100-fold by the addition of phenobarbital or one of its analogs (hexobarbital) to the growth medium. These barbiturate inducers are apparently not substrates for the enzyme nor do they activate the monooxygenase in the cell-free system. The induction efficiency of both phenobarbital and hexobarbital can be significantly increased with respect to monooxygenase activity by autoclaving the inducer in the growth medium rather than by adding it to the medium after autoclaving. Turnover numbers of about 3 000 nmoles of substrate oxygenated per min per nmole of P-450 were obtained in crude cell-free preparations obtained from maximally induced cultures. Our data indicate that products formed by heating phenobarbital or hexobarbital in the growth medium are significantly better inducers of monooxygenase activity than are the unaltered drugs.
通过在巨大芽孢杆菌ATCC 14581的生长培养基中添加苯巴比妥或其类似物之一（己巴比妥），可诱导出一种可溶性的、细胞色素P - 450依赖性脂肪酸羟化酶 - 环氧化酶复合物，其诱导倍数超过100倍。这些巴比妥类诱导剂显然不是该酶的底物，在无细胞体系中也不会激活单加氧酶。相对于单加氧酶活性，通过在生长培养基中对诱导剂进行高压灭菌，而非在高压灭菌后添加到培养基中，可显著提高苯巴比妥和己巴比妥的诱导效率。从最大诱导培养物中获得的粗无细胞制剂中，每纳米摩尔P - 450每分钟可氧化约3000纳米摩尔底物，周转数达到此值。我们的数据表明，在生长培养基中加热苯巴比妥或己巴比妥形成的产物作为单加氧酶活性的诱导剂，比未改变的药物效果要好得多。
