Kurchenko V P, Usanov S A, Metelitsa D I
Biokhimiia. 1981 Dec;46(12):2202-7.
Immunization of rats by a homogeneous cytochrome P-450-LM4 from liver microsomes of rabbits pretreated with 3-methylcholanthrene resulted in antibodies to this hemoprotein - anti-P-450-LM4. Using the Ouchterlony method, a reaction of precipitation of anti-P-450-LM4 with cytochrome P-450-LM4 from MeCh- and PB-induced microsomes of rabbit liver has been carried out. The anti-P-450-LM4 at different concentrations does not practically exert any inhibitory action on oxidation of aniline and N,N-dimethylaniline by intact and PB- and MeCh-induced rabbit hepatic microsomes. It is concluded that rabbit liver cytochrome P-450-LM4 does not practically reveal any catalytic activity in ther metabolism of aniline and N,N-dimethylaniline.
用经3-甲基胆蒽预处理的兔肝微粒体中的均一细胞色素P-450-LM4对大鼠进行免疫，产生了针对这种血红素蛋白的抗体——抗P-450-LM4。采用欧氏免疫扩散法，进行了抗P-450-LM4与来自经甲基胆蒽（MeCh）和苯巴比妥（PB）诱导的兔肝微粒体中的细胞色素P-450-LM4的沉淀反应。不同浓度的抗P-450-LM4对完整的以及经PB和MeCh诱导的兔肝微粒体氧化苯胺和N，N-二甲基苯胺几乎没有任何抑制作用。得出的结论是，兔肝细胞色素P-450-LM4在苯胺和N，N-二甲基苯胺的代谢中几乎没有显示出任何催化活性。
