Hopkin J M, Tomlinson V S, Jenkins R M
Br Med J (Clin Res Ed). 1981 Nov 7;283(6301):1209-11. doi: 10.1136/bmj.283.6301.1209.
The cytotoxic effect of cigarette smoke condensate on human polymorphs was investigated in vitro by the method of vital dye exclusion. Exposure to 1/800 of the smoke from one high-tar cigarette killed a detectable proportion of a population of 10(6) cells. The response among the cells from 40 healthy people varied widely, the percentage of dead cells ranging from 3% to 66% and from 17% to 87% at exposure levels of 125 micrograms and 250 micrograms cigarette smoke condensate respectively. Differences in individuals' responses were reproducible and unrelated to age or sex or smoking habit. The cells from 10 patients with irreversible obstructive airways disease and probable emphysema were significantly more sensitive than those from 10 patients with no respiratory disability matched for age and smoking habits. Genetically influenced variation in cellular response to cytotoxicity may be an important determinant of the risk of developing emphysema among smokers.
采用活体染料排除法在体外研究了香烟烟雾浓缩物对人多形核白细胞的细胞毒性作用。暴露于一支高焦油香烟烟雾的1/800可杀死可检测比例的10⁶个细胞群体。来自40名健康人的细胞反应差异很大，在分别暴露于125微克和250微克香烟烟雾浓缩物时，死细胞百分比范围为3%至66%和17%至87%。个体反应的差异具有可重复性，且与年龄、性别或吸烟习惯无关。10例患有不可逆性阻塞性气道疾病且可能患有肺气肿的患者的细胞比10例年龄和吸烟习惯相匹配但无呼吸功能障碍的患者的细胞明显更敏感。细胞对细胞毒性反应的遗传影响变异可能是吸烟者发生肺气肿风险的一个重要决定因素。
