Eya B K, Talcott R E
Environ Mutagen. 1980;2(3):395-404. doi: 10.1002/em.2860020310.
1-Naphthyl N-alkylcarbamates (alkyl = methyl, ethyl, n-propyl and n-butyl) were synthesized and then nitrosated with a dichloromethane extract of nitrous acid. The respective N-nitrosated carbamates were purified and tested for mutagenicity using Salmonella typhimurium LT2 strain TA98 and TA100, and Saccharomyces cerevisiae strain D3. The reactivity of the four nitrosocarbamates toward 4-(p-nitrobenzyl)-pyridine (NBP) was measured in order to assess their strengths as alkylating agents. Bioassay results indicated an inverse relationship between the length of the N-alkyl chain and the mutagenicity of the N-nitrosonaphthylcarbamates. The NBP test results indicated an inverse relationship between chemical reactivity and N-alkyl chain length.
合成了1-萘基N-烷基氨基甲酸酯（烷基 = 甲基、乙基、正丙基和正丁基），然后用亚硝酸的二氯甲烷提取物对其进行亚硝化。对各自的N-亚硝化氨基甲酸酯进行纯化，并使用鼠伤寒沙门氏菌LT2菌株TA98和TA100以及酿酒酵母菌株D3测试其致突变性。测定了四种亚硝基氨基甲酸酯对4-(对硝基苄基)-吡啶（NBP）的反应活性，以评估它们作为烷基化剂的强度。生物测定结果表明，N-烷基链的长度与N-亚硝基萘基氨基甲酸酯的致突变性之间呈反比关系。NBP测试结果表明，化学反应性与N-烷基链长度之间呈反比关系。
