Stenberg E, Styrvold O B, Strøm A R
J Bacteriol. 1982 Jan;149(1):22-8. doi: 10.1128/jb.149.1.22-28.1982.
Cells of Proteus sp. strains NTHC153 grown anaerobically with glucose and trimethylamine oxide (TMAO) were converted to spheroplasts by the penicillin method. The spheroplasts were lysed by osmotic shock, and the membrane vesicles were purified by sucrose gradient centrifugation. Vesicles energized electron transfer from formate to TMAO displayed active anaerobic transport of serine. An anaerobic cell-free extract of Proteus sp. disrupted in a French pressure cell reduced TMAO with formate and NADH with the concomitant formation of organic phosphate. The net P/2e- ratios determined were 0.1 and 0.3, respectively. The NADH- and TMAO-dependent phosphorylation was sensitive to uncouplers of oxidative phosphorylation (protonophores), and the formate- and TMAO-dependent serine transport was sensitive to ionophores and protonophores. We conclude that TMAO reduction in Proteus sp. fulfills the essential features of anaerobic respiration.
奇异变形杆菌（Proteus sp.）菌株NTHC153的细胞在以葡萄糖和氧化三甲胺（TMAO）为底物进行厌氧培养后，通过青霉素法转化为原生质球。原生质球经渗透压休克裂解，膜囊泡通过蔗糖梯度离心法纯化。能使电子从甲酸转移至TMAO的囊泡表现出对丝氨酸的活跃厌氧转运。在法国压榨器中破碎得到的奇异变形杆菌厌氧无细胞提取物，能利用甲酸还原TMAO，并利用NADH还原TMAO，同时伴随有机磷酸盐的形成。测定得到的净P/2e-比值分别为0.1和0.3。依赖NADH和TMAO的磷酸化对氧化磷酸化解偶联剂（质子载体）敏感，而依赖甲酸和TMAO的丝氨酸转运对离子载体和质子载体敏感。我们得出结论，奇异变形杆菌中TMAO的还原符合厌氧呼吸的基本特征。
