Rundegren J, Ericson T
J Oral Pathol. 1981 Aug;10(4):269-75. doi: 10.1111/j.1600-0714.1981.tb01273.x.
Using a serotype c strain of Streptococcus mutans we found that the agglutinating activity could be quantitatively absorbed, and desorbed from the micro-organisms by a phosphate buffer. The desorbed agglutinin was found to be inactive in the absence of calcium. Calcium alone did not induce aggregation. At 0.1 mM calcium most of the activity was regained. Other cations could not substitute for calcium. On a molecular basis the ratio between calcium and agglutinin or bacteria is high, in the order of 10(7) and 10(4) respectively.
使用变形链球菌血清型c菌株，我们发现凝集活性可以被定量吸收，并通过磷酸盐缓冲液从微生物中解吸出来。发现解吸的凝集素在没有钙的情况下没有活性。单独的钙不会诱导聚集。在0.1 mM钙时，大部分活性得以恢复。其他阳离子不能替代钙。从分子层面来看，钙与凝集素或细菌之间的比例很高，分别约为10⁷和10⁴。
