Reinhardt D J, Licata I, Kaplan W, Ajello L, Chandler F W, Ellis J J
Sabouraudia. 1981 Dec;19(4):245-56. doi: 10.1080/00362178185380421.
We investigated the potential of 33 different zygomycete isolates to cause cerebral disease following the intranasal instillation of their spores into ketotic rabbits with alloxan induced diabetes. The isolates represented six thermotolerant species of Rhizopus (R. arrhizus, R. chinensis, R. microsporus, R. oligosporus, R. oryzae, and R. rhizopodiformis), Absidia corymbifera, Cunninghamella bertholletiae, and Rhizomucor pusillus. All 13 isolates of the thermotolerant Rhizopus species proved to be cerebral pathogens as confirmed by culture and histopathology. One isolate of R. oligosporus and one isolate of R. rhizopodiformis, however, were less pathogenic than isolates of other Rhizopus species tested. Cerebral pathogenicity was noted with 2 of 5 isolates of Rh. pusillus and only 1 of 13 A. corymbifera isolates. Two thermotolerant C. bertholletiae cultures, recovered from human lesions, did not cause either cerebral or pulmonary disease in ketotic rabbits. The incidence of pulmonary zygomycosis caused by the isolates of the species of the four genera under study was as follows: Rhizomucor 24%, Rhizopus 22%, Absidia 9%, and Cunninghamella 0%. This study confirms the pathogenic potential of the thermotolerant species of Rhizopus to cause cerebral zygomycosis in ketotic diabetic rabbits and also revealed the potential of Rh. pusillus and A. corymbifera occasionally to cause the same disease in animals and humans.
我们通过将33种不同接合菌的孢子经鼻内滴注到用四氧嘧啶诱导糖尿病的酮病兔体内，研究了它们引发脑部疾病的可能性。这些分离株代表了根霉属的6种耐热菌种（少根根霉、华根霉、微小根霉、少孢根霉、米根霉和类根根霉）、伞枝犁头霉、柏氏小克银汉霉和米根毛霉。经培养和组织病理学证实，所有13株耐热根霉属菌种的分离株均为脑部病原体。然而，少孢根霉的1个分离株和类根根霉的1个分离株的致病性低于所测试的其他根霉属菌种的分离株。在米根毛霉的5个分离株中有2个以及在伞枝犁头霉的13个分离株中仅有1个被发现具有脑部致病性。从人类病变中分离出的2株耐热柏氏小克银汉霉培养物，在酮病兔中未引发脑部或肺部疾病。所研究的四个属的菌种分离株引起肺部接合菌病的发生率如下：米根毛霉24%，根霉22%，犁头霉9%，小克银汉霉0%。本研究证实了耐热根霉属菌种在酮病糖尿病兔中引发脑部接合菌病的致病潜力，同时也揭示了米根毛霉和伞枝犁头霉偶尔在动物和人类中引发相同疾病的可能性。
