Baron S S, Rowe J J
Antimicrob Agents Chemother. 1981 Dec;20(6):814-20. doi: 10.1128/AAC.20.6.814.
Biologically produced pyocyanin was purified, and the nature of its antibacterial action was determined for several bacteria. The pigment was shown to be bactericidal for all susceptible organisms. The bactericidal effect was dependent upon pyocyanin concentration and resulted in decreases in viability ranging from 1 to 8 log viable cells ml-1. The gram-positive bacteria were more susceptible as a group to the antibiotic action than were the gram-negative bacteria. All apyocyanogenic pseudomonads tested were totally resistant to the pigment, suggesting that resistance may be a characteristic of the genus. Pseudomonas aeruginosa, the producer organism, was also essentially unaffected by high concentrations of pyocyanin. Facultative anaerobes were twofold or more times resistant to the action of the pigment under fermentative conditions; however, the antibiotic action did not require oxygen since denitrifying bacteria were more susceptible during anaerobic respiration than during aerobic respiration.
对生物合成的绿脓菌素进行了纯化，并测定了其对几种细菌的抗菌作用性质。结果表明，该色素对所有敏感生物均具有杀菌作用。杀菌效果取决于绿脓菌素的浓度，可使活菌数减少1至8个对数单位/毫升。革兰氏阳性菌作为一个群体比革兰氏阴性菌对抗生素作用更敏感。所有测试的无绿脓菌素产生的假单胞菌对该色素完全耐药，这表明耐药性可能是该属的一个特征。产生该色素的铜绿假单胞菌对高浓度的绿脓菌素也基本无影响。兼性厌氧菌在发酵条件下对该色素的作用具有两倍或更高的耐药性；然而，抗生素作用并不需要氧气，因为反硝化细菌在无氧呼吸过程中比在有氧呼吸过程中更敏感。
