Biagianti S, Corsin J
Arch Anat Microsc Morphol Exp. 1981;70(2):91-107.
Sodium hyaluronate, present in large quantities during cellular multiplication and the migration phase, is not found in the extracellular matrix during early chondrocyte differentiation. Microinjections enabled a higher concentration of hyaluronate to be maintained than in normal embryos. This leads to the appearance of anomalies in the cartilage structure; these are always reversible and more or less marked according to the amount of hyaluronate injected and to the timing of the injection. If the injection is made as soon as differentiation takes place, or after it, a decrease of chondroïtine-sulfate excretion is observed. As these molecules are responsible for the differentiated state of chondrocytes, hyaluronate appears to be an extracellular substance that regulates chondrogenesis.
透明质酸钠在细胞增殖和迁移阶段大量存在，而在软骨细胞早期分化过程中，细胞外基质中并未发现。显微注射能够维持比正常胚胎中更高浓度的透明质酸盐。这会导致软骨结构出现异常；这些异常总是可逆的，并且根据注射的透明质酸盐量和注射时间，或多或少会比较明显。如果在分化一开始或之后立即进行注射，会观察到硫酸软骨素排泄减少。由于这些分子负责软骨细胞的分化状态，透明质酸盐似乎是一种调节软骨形成的细胞外物质。
