Kato Y, Mukudai Y, Okimura A, Shimazu A, Nakamura S
Department of Biochemistry, School of Dentistry, Hiroshima University, Japan.
J Rheumatol Suppl. 1995 Feb;43:158-9.
Hyaluronic acid (HA) of large sizes suppressed the release of cartilage matrix proteoglycan, fibronectin, and other macromolecules from the cell matrix layer of chondrocyte cultures, perhaps because HA of large sizes formed a viscous barrier in the matrix by its interactions with other extracellular matrix macromolecules. To test this possibility, we determined the viscosity of solutions containing HA of various sizes in the presence of proteoglycan monomer or chondroitin sulfate glycosaminoglycan (GAG). Not only the monomer but also chondroitin sulfate increased the viscosity of HA solutions, depending on the size of HA. These findings suggest that HA of large sizes increases the viscosity near the surface of articular cartilage by sugar-sugar and by sugar-protein interactions and that the increase of viscosity is involved in the protective action of HA on arthritic cartilage.
大尺寸透明质酸（HA）抑制了软骨细胞培养物细胞基质层中软骨基质蛋白聚糖、纤连蛋白和其他大分子的释放，这可能是因为大尺寸HA通过与其他细胞外基质大分子相互作用在基质中形成了粘性屏障。为了验证这种可能性，我们测定了在蛋白聚糖单体或硫酸软骨素糖胺聚糖（GAG）存在下含有各种尺寸HA的溶液的粘度。不仅单体，而且硫酸软骨素都增加了HA溶液的粘度，这取决于HA的尺寸。这些发现表明，大尺寸HA通过糖-糖和糖-蛋白相互作用增加了关节软骨表面附近的粘度，并且粘度的增加参与了HA对关节炎软骨的保护作用。
