Robertson I G, Birnbaum L S
Chem Biol Interact. 1982 Jan;38(2):243-52. doi: 10.1016/0009-2797(82)90043-6.
Age-related changes in drug metabolism of the liver, lung and kidney of adult female Long-Evans rats were determined by measuring changes in mutagen formation. Activation of aflatoxin B1 (AFB1), 2-aminofluorene (AF) and 2-acetylaminofluorene (AAF) to mutagenic derivatives was assayed using the Ames Salmonella test system. The promutagens were incubated with tissue fractions from rats ranging in age from 2.5 to 25 months. With all three compounds, hepatic, renal and pulmonary activation was lower in the senescent than in the young adult animals. The largest decrease, however, occurred prior to middle-age, i.e. before 9-13 months. In liver and kidney, little change was detectable between the middle-aged and the old (20-25 months) animals. However, pulmonary metabolism in the oldest animals was slightly higher than in the extracts from the middle-aged rats. The observed decline in mutagen activation may thus be a function of maturation rather than senescence.
通过测量诱变剂形成的变化，确定成年雌性Long-Evans大鼠肝脏、肺和肾脏中与年龄相关的药物代谢变化。使用Ames沙门氏菌测试系统检测黄曲霉毒素B1（AFB1）、2-氨基芴（AF）和2-乙酰氨基芴（AAF）向诱变衍生物的活化情况。将前诱变剂与年龄在2.5至25个月之间的大鼠组织匀浆一起孵育。对于所有这三种化合物，衰老动物肝脏、肾脏和肺的活化作用均低于年轻成年动物。然而，最大的下降发生在中年之前，即9至13个月之前。在肝脏和肾脏中，中年动物和老年（20至25个月）动物之间几乎没有可检测到的变化。然而，最老动物的肺代谢略高于中年大鼠的提取物。因此，观察到的诱变剂活化下降可能是成熟而非衰老的作用。
