Brouns R M, van Doorn R, Bos R P, Mulleners L J, Henderson P T
Toxicology. 1981;19(1):67-75. doi: 10.1016/0300-483x(81)90066-4.
The aromatic amine 2-aminofluorene (2-AF) is metabolised by isolated rat liver cells to reactive species, thereby causing mutagenic effects in Salmonella typhimurium TA 1538 and evoking DNA-excision repair within the liver cells. The pathway leading to the production of metabolites mutagenic in Salmonella is likely to proceed via direct N-hydroxylation of 2-AF to N-hydroxy-2-aminofluorene (N-OH-2-AF). On the other hand, the formation of intermediates giving rise to hepatocellular DNA-repair is shown to depend upon N-acetylation of 2-AF to 2-acetylaminofluorene(2-AAF), whereas a subsequent conjugation reaction, most likely to be sulfate ester formation, is also essentially involved.
芳香胺2-氨基芴（2-AF）被分离的大鼠肝细胞代谢为活性物质，从而在鼠伤寒沙门氏菌TA 1538中产生致突变作用，并在肝细胞内引发DNA切除修复。导致沙门氏菌中产生致突变代谢物的途径可能是通过2-AF直接N-羟基化生成N-羟基-2-氨基芴（N-OH-2-AF）。另一方面，导致肝细胞DNA修复的中间体的形成显示取决于2-AF N-乙酰化为2-乙酰氨基芴（2-AAF），而随后的结合反应，最有可能是硫酸酯形成，也基本参与其中。
